Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分的蛋白经过镍住得到进一步的化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用化的山羊痘病毒分别免疫家兔豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分化培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理化后卖给消费者，其价格常常翻一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文杨梅红素的提取、化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,分化柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终化产物为一条染带，化倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-子交换谱分步洗脱法,对牛初乳中的乳过氧化物酶进行分化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲层析柱(Ni-NTA agarose)化凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分、化、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、子交换谱、(NH4)2SO4沉淀亲谱法进行化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化为N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使化的生物分子结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术化祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分化方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对化的蛇毒制品非化的蛇毒制品已有较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍从酵母中分化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法化学渗透法,并对其进行对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分化方法,并研究其酶学性质。方法采用子交换层析直接将木瓜凝乳蛋白酶分化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用纯化的山羊痘病毒分别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离纯化培养无黑。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯化后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红的提取、纯化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,分离纯化柱晶白霉。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯化产物为一条染带，纯化倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱分步洗脱法,对牛初乳中的乳过氧化物酶进行了分离和纯化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后，ELISA结果证实其有好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、纯化、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行纯化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化为N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分离纯化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使纯化的生物分子结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术纯化祖师麻乙醇提取液,祖师麻甲保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯化的蛇毒制品和非纯化的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香纯化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离纯化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶分离纯化,通过温度、葡萄糖、盐酸胍及其他因研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要纯化的山羊痘病毒分别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离纯化培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯化后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提、纯化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

大孔网状树脂吸附法代替溶法,分离纯化柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯化产物一条染带，纯化倍数144.83，回收率38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

超滤-离子交换谱分步洗脱法,对牛初乳中的乳过氧化物酶进行了分离和纯化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母原料,经菠萝蛋白酶酶解、分离、纯化、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行纯化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基缩酮保护,再正己烷重结晶分离纯化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运是基于首先使纯化的生物分子结晶有序排列然后X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果超滤技术纯化祖师麻乙醇提液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其体外溶核作。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯化的蛇毒制品和非纯化的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

松香纯化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离纯化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其酶学性质。方法离子交换层析直接将木瓜凝乳蛋白酶分离纯化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离蛋白经过镍住得到了进一步纯。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用纯山羊痘病毒分别免疫家兔和豚鼠，制备高效价抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目从人头皮毛囊外根鞘分离纯培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯后卖给消费者，价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素提取、纯理性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,分离纯柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯产物为一条染带，纯倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱分步洗脱法,对牛初过氧物酶进行了分离和纯。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯和凝胶柱上在位复性后，ELISA结果证实具有良好结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、纯、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行纯。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯后催活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转为N-乙酰-D-神经氨酸,表现出较高催活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分离纯。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法运用是基于首先使纯生物分子结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术纯祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效木瓜凝蛋白酶分离纯方法,并研究体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯蛇毒制品和非纯蛇毒制品已有了较深入研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香纯得到松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母分离纯谷胱甘肽(GSH)两种抽提方法:对羟基苯甲酸丙酯法和学渗透法,并对进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目建立简单有效木瓜凝蛋白酶分离纯方法,并研究酶学性质。方法采用离子交换层析直接将木瓜凝蛋白酶分离纯,通过温度、葡萄糖、盐酸胍他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经镍住得到了进一步的。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用的山羊痘病毒分别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经处理和后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的、及其理性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃法,分离柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终产物为一条染带，倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱分步洗脱法,对牛初乳中的乳物酶进行了分离和。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经后催活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转为N-乙酰-D-神经氨酸,表现出较高的催活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分离。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使的生物分子结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术祖师麻乙醇液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分离方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对的蛇毒制品和非的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离谷胱甘肽(GSH)的两种抽方法:对羟基苯甲酸丙酯法和学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分离方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶分离,通温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用的山羊痘病毒分别免疫家兔豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提取、及其理性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,分离柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终产物为一条染带，倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱分步洗脱法,对牛初乳中的乳过氧物酶进行了分离。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲层析柱(Ni-NTA agarose)凝胶柱上性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀亲谱法进行。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经后催活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转为N-乙酰-D-神经氨酸,表现出较高的催活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分离。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使的生物分子结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分离方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对的蛇毒制品非的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分离方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶分离,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋经过镍住得到了进一步的。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用的山羊痘病毒分别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提取、及其理性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,分离柱素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终产物为一条染，倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱分步洗脱法,对牛初乳中的乳过氧物酶进行了分离和。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋酶酶解、分离、、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经后催活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转为N-乙酰-D-神经氨酸,表现出较高的催活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结分离。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使的生物分子结为有序排列然后用X射线分析结体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋酶分离方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对的蛇毒制品和非的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋酶分离方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋酶分离,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用纯化的山羊痘病毒分别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘分离纯化培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯化后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提取、纯化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂法代替溶媒萃取法,分离纯化柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯化产物为一条染带，纯化倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离谱分步洗脱法,对牛初乳中的乳过氧化物酶进行了分离和纯化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、纯化、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离谱、(NH4)2SO4沉淀和亲和谱法进行纯化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化为N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶分离纯化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使纯化的生物分结晶为有序排列然后用X射线分析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术纯化祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯化的蛇毒制品和非纯化的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香纯化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中分离纯化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分离纯化方法,并研究其酶学性质。方法采用离层析直接将木瓜凝乳蛋白酶分离纯化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质离的蛋白经过镍住得到了一步的纯化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用纯化的山羊痘病毒别免疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘离纯化培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯化后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提取、纯化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,离纯化柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯化产物为一条染带，纯化倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱步洗脱法,对牛初乳中的乳过氧化物酶了离和纯化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以啤酒废酵母为原料,经菠萝蛋白酶酶解、离、纯化、干燥等工序制得啤酒废酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法纯化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化为N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶离纯化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使纯化的生物子结晶为有序排列然后用X射线析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术纯化祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶离纯化方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯化的蛇毒制品和非纯化的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香纯化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中离纯化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶离纯化方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶离纯化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间离的蛋白经过镍住得到了进一步的纯化。

The antisera against purified goatpox virus were obtained from rabbits and guinea pigs.

摘要用纯化的山羊痘病毒疫家兔和豚鼠，制备高效价的抗血清。

Objective: To isolate and culture hair outer root sheath amelanotic melanocyte derived from human scalp.

目的从人头皮毛囊外根鞘离纯化培养无素黑素细胞。

The water is treated, purified and sold to us, often at a thousandfold increase in price.

这些水经过处理和纯化后卖给消费者，其价格常常翻了一千倍。

In this paper, the extraction depurate and physico-chemical characterization of red Pigment for myrica rubra S.et Z. were developed.

本文报道了杨梅红素的提取、纯化及其理化性质。

The macroreticular resin to adsorb leucomycin( LM) as the better method to replace the solvent extraction method was designed.

用大孔网状树脂吸附法代替溶媒萃取法,离纯化柱晶白霉素。

Relative activity was 144.83.And recovery was 38.66%.Conclusion:The industrial feature was reflected in the purification process of thrombolysin.

聚丙烯酰胺凝胶电泳考马斯亮蓝染法结合显示最终纯化产物为一条染带，纯化倍数为144.83，回收率为38.66％。

A stepwise procedure for isolation and purification of lactoperoxidase by ultrafiltration and ion exchange chromatography was investigated.

采用超滤-离子交换谱步洗脱法,对牛初乳中的乳过氧化物酶进行了离和纯化。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。

Waste beer yeast polypeptides were made from waste beer yeast by bromidic hydrolyzation, separation, purification and dryness etc.

以酵母为原料,经菠萝蛋白酶酶解、离、纯化、干燥等工序制得酵母活性肽。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换谱、(NH4)2SO4沉淀和亲和谱法进行纯化。

The enzyme activity test results showed that aldonase could transform Nacetyl-D-ManNAc into N-acetyl-D-neuraminic acid very efficiently.

经纯化后催化活性实验结果表明,醛缩酶可将N-乙酰-D-甘露糖胺高效率地转化为N-乙酰-D-神经氨酸,表现出较高的催化活性。

METHODS The hydroxyl groups of magnolol were protected using ketol reaction and the products were purified by recrystallization from hexane.

方法将厚朴酚两羟基用缩酮保护,再用正己烷重结晶离纯化。

This is achieved by first crystallizing the purified biomolecule into ordered arrays and then using X-ray diffraction to analyze the crystals.

这种方法的运用是基于首先使纯化的生物子结晶为有序排列然后用X射线析结晶体。

Results The reserved rate of aphnetin was over 96 %,the ability of purification was strong and the ultrafiltrate was very pellucid.

结果用超滤技术纯化祖师麻乙醇提取液,祖师麻甲素保留率可达96%以上,滤液澄明度好,除杂力度较大。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶离纯化方法,并研究其体外溶核作用。

In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin.

人们对纯化的蛇毒制品和非纯化的蛇毒制品已有了较深入的研究。

From rosin purified abietic acid was prepared by using abietic acid as stared material acylamino surfactant was synthesized by maleric anhydride and diethanolamine.

用松香纯化得到的松香酸(枞酸)与马来酸酐反应得马来海松酸,再与二乙醇胺反应制得松香马来酰胺基乙醇表面活情剂.

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione(GSH) from yeast have been introduced and compared in this review.

介绍了从酵母中离纯化谷胱甘肽(GSH)的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其进行了对比。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶离纯化方法,并研究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶离纯化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。