Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入胞后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体胞与胞电融合时可通过增加PVS中体胞的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在胞黄形成期，它的RNA和DNA的合成均较快，因此认为滤泡上皮胞合成分泌了一些物质参与黄蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级胞又分裂成两个大小不对等的新胞：一个相对很大的子（成熟胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入卵母胞后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低效率的体胞与卵母胞电通过增加PVS中体胞的数目来提效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在卵母胞卵黄形成期，它的RNA和DNA的成均较快，因此认为滤泡上皮胞成分泌了一些物质参与卵黄蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此，次级卵母胞又分裂成两个大小不对等的新胞：一个相对很大的卵子（成熟卵胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立受精的方法，并探讨小鼠附睾精子注射进入卵母胞后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体胞与卵母胞电融合时可通过增加PVS中体胞的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而皮胞卵母胞卵黄形成期，它的RNA和DNA的合成均较快，因此认为皮胞合成分泌了一些物质参与卵黄蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级卵母胞又分裂成两个大小不对等的新胞：一个相对很大的卵子（成熟卵胞）和一个小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子显微注射进入卵母后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的与卵母电融合时可通过增加PVS中的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮卵母卵黄形成期，它的RNA和DNA的合成均较快，因此认为滤泡上皮合成分泌了一些物质参与卵黄蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级卵母又分裂成两个大小不对等的新：一个相对很大的卵子（成熟卵）和一个微小的次级极。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入卵母胞后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

融合效率的体胞与卵母胞电融合时可通过增加PVS中体胞的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在卵母胞卵期，它的RNA和DNA的合均较快，因此认为滤泡上皮胞合分泌了一些物质参与卵蛋白的。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级卵母胞又分裂两个大小不对等的新胞：一个相对很大的卵子（熟卵胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目:建立显微受精方法，并探讨小鼠附睾精子显微注射进入母后受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率与母电融合时可通过增加PVS中数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮母黄形成期，它RNA和DNA合成均较快，因此认为滤泡上皮合成分泌了一些物质参与黄蛋白形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级母又分裂成两个大小不对等新：一个相对很大子（成熟）和一个微小次级极。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立显微受精的方法，并探讨小鼠附睾精子在显微入卵母胞后的受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体胞与卵母胞电融合时可通过增加PVS中体胞的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在卵母胞卵黄形成期，它的RNA和DNA的合成均较快，因滤泡上皮胞合成分泌了一些物质参与卵黄蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

时，次级卵母胞又分裂成两个大小不对等的新胞：一个相对很大的卵子（成熟卵胞）和一个微小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目的:建立精的方法，并探讨小鼠附睾精子在注射进入母胞后的精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体胞母胞电融合时可通过增加PVS中体胞的数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在母胞形成期，它的RNA和DNA的合成均较快，因此认为滤泡上皮胞合成分泌了一些物质参蛋白的形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级母胞又分裂成两个大小不对等的新胞：一个相对很大的子（成熟胞）和一个小的次级极体。

Abstract： Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要： 目:建立显微受精法，并探讨小鼠附睾精子在显微注射进入卵母胞后受精能力。

Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率体胞与卵母胞电融合时可通过增加PVS中体胞数目来提高融合效率。

Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

而滤泡上皮胞在卵母胞卵黄形成期，它RNA和DNA合成均较快，因此认为滤泡上皮胞合成分泌了一参与卵黄蛋白形成。

The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时，次级卵母胞又分裂成两个大小不对等新胞：一个相对很大卵子（成熟卵胞）和一个微小次级极体。